Journal: Developmental Cell

Article Title: Defining the identity and the niches of epithelial stem cells with highly pleiotropic multilineage potency in the human thymus

doi: 10.1016/j.devcel.2023.08.017

Figure Lengend Snippet:

Article Snippet: HumanKine® recombinant human FLT3 Ligand protein , Proteintech , cat# HZ-1151.

Techniques: Recombinant, Modification, Selection, RNA Extraction, Polymer, Cell Culture, Derivative Assay, Software

Journal: Leukemia

Article Title: In vivo treatment of mutant FLT3-transformed murine leukemia with a tyrosine kinase inhibitor.

doi: 10.1038/sj.leu.2401680

Figure Lengend Snippet: Figure 1 (a) IL3-independent proliferation of 32D cells transfected with mutant FLT3 gene. (b) An inhibitory effect of herbimycin A on the in vitro growth of parental 32D cells and mutant FLT3-transfected 32D (mFLT3/32D) cells. Herbimycin A was added to the culture at indicated concentrations, and viable cells were counted by trypan blue dye-exclusion assay after 72 h culture.

Article Snippet: Recombinant human FLT3 ligand (FL) was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Transfection, Mutagenesis, In Vitro, Exclusion Assay

Journal: Leukemia

Article Title: In vivo treatment of mutant FLT3-transformed murine leukemia with a tyrosine kinase inhibitor.

doi: 10.1038/sj.leu.2401680

Figure Lengend Snippet: Figure 2 Inhibition of the autophosphorylation of mutant FLT3 by herbimycin A. Cos7 cells were transfected with wild-type and mutant FLT3 cDNA After 72 h culture, the cells were treated with herbimycin at 0.1, 0.5 and 1.0 nM in combination with or without FL at 50 ng/ml for 3 h. Cell lysates were immunoprecipitated by anti-FLT3 antibody and immunoblotted by anti-phosphotyrosine antibody.

Article Snippet: Recombinant human FLT3 ligand (FL) was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Inhibition, Mutagenesis, Transfection, Immunoprecipitation